anti cd68 primary antibody  (Bioss)

Journal: Journal of Cardiovascular Translational Research

Article Title: Extension of Atherosclerosis ApoE-/- Mouse—a Model of Chronic Myocardial Ischemia and Evaluation Method

doi: 10.1007/s12265-025-10734-8

Figure Lengend Snippet: A HE: It can be seen that the cardiomyocytes in the model group are significantly enlarged and the nuclei become sparse. Sirius red staining: Some areas of myocardium in the model group showed an increase in cell spacing accompanied by fibrotic tissue metaplasia. In contrast, myocytes in the control group were neatly arranged and densely distributed, with significantly less fibrous tissue than in the model group. “LV”: left ventricle. “RV”: right ventricle. B In the model group, a small number of apoptotic cells were observed with green fluorescence, but no large aggregates were detected. C Additionally, there were numerous CD68-positive cells expressing red fluorescence, which were irregularly distributed on the cell wall and in the cytoplasm

Article Snippet: Tissue sections underwent blocking with 5% bovine serum albumin (BSA) (Sigma-Aldrich Cat# A7906) in PBST (0.1% Tween-20 in PBS) for 1 h at room temperature, followed by overnight incubation at 4 °C with anti-CD68 primary antibody (Rabbit polyclonal, Bioss Antibodies, Cat# bs-20403R, RRID: AB_10855800) diluted 1:100 in blocking solution.

Techniques: Staining, Control, Fluorescence, Expressing

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Single-cell sequencing and network pharmacology coupled with molecular docking and experimental validation reveal the effects of YPFS on macrophages in stage I non-small cell lung cancer

doi: 10.1007/s00262-026-04305-2

Figure Lengend Snippet: Pharmacology Network Analysis and Target Screening of YPFS. A Venn diagram depicting the overlap among survival-related genes, YPFS targets, and macrophage-associated genes, identifying AHSA1 as the intersection gene. B Network diagram showing relationships between active compounds and target genes. Green nodes represent target genes; red nodes, compounds from largehead atractylodes root; blue nodes, compounds from milkvetch root; and yellow nodes, compounds from divaricate saposhniovia root. C Interaction diagram illustrating AHSA1 and its targeting interactions with wogonin, kaempferol, and quercetin. D Kaplan–Meier survival curves of NSCLC patients stratified by high and low AHSA1 expression, showing poorer prognosis in the high-expression group. E Violin plot depicting AHSA1 expression across different cell types in single-cell data, with highest expression in epithelial cells. F Violin plot showing AHSA1 expression across macrophage functional subtypes, with peak expression observed in MAC3. G Violin plot comparing AHSA1 expression levels in macrophages from normal versus tumor tissues. H Tissue microarray immunofluorescence staining of AHSA1 and CD68 in normal and tumor tissues. Scale bar, 50 μm

Article Snippet: Staining was performed with the Tyramide Signal Amplification (TSA) kit (ABclonal, CAT #RK05902) using primary antibodies against CD68 (ABclonal, CAT #A20803) and AHSA1 (Invitrogen, CAT #PA5-27672), according to the manufacturer’s instructions.

Techniques: Expressing, Functional Assay, Microarray, Immunofluorescence, Staining